Defective postsecretory maturation of MUC5B mucin in cystic fibrosis airways

In cystic fibrosis (CF), airway mucus becomes thick and viscous, and its clearance from the airways is impaired. The gel-forming mucins undergo an ordered "unpacking/maturation" process after granular release that requires an optimum postsecretory environment, including hydration and pH. We hypothesized that this unpacking process is compromised in the CF lung due to abnormal transepithelial fluid transport that reduces airway surface hydration and alters ionic composition. Using human tracheobronchial epithelial cells derived from non-CF and CF donors and mucus samples from human subjects and domestic pigs, we investigated the process of postsecretory mucin unfolding/maturation, how these processes are defective in CF airways, and the probable mechanism underlying defective unfolding. First, we found that mucins released into a normal lung environment transform from a compact granular form to a linear form. Second, we demonstrated that this maturation process is defective in the CF airway environment. Finally, we demonstrated that independent of HCO3- and pH levels, airway surface dehydration was the major determinant of this abnormal unfolding process. This defective unfolding/maturation process after granular release suggests that the CF extracellular environment is ion/water depleted and likely contributes to abnormal mucus properties in CF airways prior to infection and inflammation

A finite characterization of K -matrices in dimensions less than four

The class of real n × n matrices M , known as K -matrices, for which the linear complementarity problem w − Mz = q, w ≥ 0, z ≥ 0, w T z =0 has a solution whenever w − Mz =q, w ≥ 0, z ≥ 0 has a solution is characterized for dimensions n <4. The characterization is finite and ‘practical’. Several necessary conditions, sufficient conditions, and counterexamples pertaining to K -matrices are also given. A finite characterization of completely K -matrices ( K -matrices all of whose principal submatrices are also K -matrices) is proved for dimensions <4.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47913/1/10107_2005_Article_BF01589438.pd

Perspectives in visual imaging for marine biology and ecology: from acquisition to understanding

Durden J, Schoening T, Althaus F, et al. Perspectives in Visual Imaging for Marine Biology and Ecology: From Acquisition to Understanding. In: Hughes RN, Hughes DJ, Smith IP, Dale AC, eds. Oceanography and Marine Biology: An Annual Review. 54. Boca Raton: CRC Press; 2016: 1-72

Predicting Affordances from Gist

Abstract. This paper presents an incremental learning mechanism to create associations between the affordances provided by the environment and its gist. The proposed model aims at helping the agent on the prioritisation of its perceptual resources, and consequently on visual attention. The focus on affordances, rather than on objects, enables a self-supervised learning mechanism without assuming the existence of symbolic object representations, thus facilitating its integration on a developmental framework. The focus on affordances also contributes to our understanding on the role of sensorimotor coordination on the organisation of adaptive behaviour. Promising results are obtained with a physical experiment on a natural environment, where a camera was handled as if it was being carried by an actual robot performing obstacle avoidance, trail following and wandering behaviours.

The polygenetic Kahouanne Seamounts in the northern Lesser Antilles island arc: evidence for large-scale volcanic island subsidence

Remotely operated vehicle (ROV) explorations of the Kahouanne Seamounts, located 25 km SE of Montserrat in the northern Lesser Antilles island arc, have discovered the occurrence of honeycomb-scalloped erosional features on volcanic and limestone outcrops at depths of up to 600 m below sea level (mbsl). These features, combined with the flat-topped morphology of the seamounts, the occurrence of shallow-water carbonates (rhodoliths, benthic foraminifera), and the presence of oxidized, highly-vesicular volcanic fragments, suggest that the seamounts were once subaerial islands and have subsequently subsided to their present depth. The subsidence is likely to have been in response to a combination of 1) graben development southeast of the island of Montserrat where faulting and extension are driven by the accommodation of slip convergence associated with oblique subduction along the Lesser Antilles volcanic arc and 2) regional subsidence inferred from studies of nearby carbonate platforms. 40Ar/39Ar dating of basalt/andesite lavas from the central seamount indicates active volcanism around 5.4 Ma. Shallow-water carbonates recovered from an apparent wave-cut terrace at ~600 mbsl in the same area, were deposited approximately 3 Ma, based on Sr-isotope stratigraphy, indicating significant subsidence of the complex since that time. The total subsidence is in line with displacements in the Kahouanne valley graben and regional subsidence rates inferred from carbonate platform depths around the islands of Guadeloupe and Martinique, implying that intra-arc subsidence has been a significant process shaping the present bathymetry and topography of this island arc